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GHK-Cu Reconstitution & Dose Calculator

GHK-Cu is a copper-binding tripeptide studied for skin, collagen, and hair-follicle support. It ships in much larger vials than most peptides, commonly 50, 100, or 200 mg. This free calculator turns a GHK-Cu vial plus reconstitution solution (bacteriostatic water) into insulin syringe units to draw. It is unit-conversion math, not a dosing recommendation. GHK-Cu vials are commonly 50 mg, 100 mg, 200 mg, so the tool below starts at 50 mg; change any field to match your own vial.

No single volume of reconstitution solution is correct for a 50 mg GHK-Cu vial: the amount added sets the concentration. Concentration equals vial milligrams divided by millilitres added — 50 mg in 1 mL gives 50 mg/mL, in 2 mL 25 mg/mL. Syringe units follow from that concentration.

Free tool

Reconstitution calculator

Pick your peptide, vial size, and BAC water. Get exact units to draw on a 1 mL insulin syringe.

Educational reconstitution math reference only. This tool does not provide dosage or usage recommendations. Consult a qualified healthcare provider before making any health decisions.
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Your mix
What you mixed
5 mg powder + 2 mL water
Concentration of the liquid
250 mcg per 10 units
Each small line on a 1 mL insulin syringe = 25 mcg
One 5 mg vial lasts you
20days2.9 weeks
At 250 mcg, 1 dose / day, 20 total doses in this vial.
Insulin syringe, draw to the 10 unit mark10 u
0102030405060708090100
Pull the plunger back until the rubber stopper lines up with the 10 mark on the barrel. The red liquid in the picture is the peptide volume.
Quick checks
How long will it last? About 20 doses, or 2.9 weeks at daily.
What line do I draw to? Draw to 10 units on a 1 mL insulin syringe. If that number is tiny, more BAC water makes the unit draw easier to read.
mg vs mcg: The dose you entered is 250 mcg, which is 0.250 mg. Enter doses here in mcg.
Blend labels: For total-vial duration, use the total powder in the vial. If the label lists 5 mg + 5 mg, that is 10 mg total. For per-component math, run each component from its own labeled mg.
Syringe unit referenceFull guide →
  • • Standard insulin syringe = 100 units total
  • • Small markings = 1 unit each
  • • Large markings = 10 units (0.1 mL)
  • 100 units = 1 mL

Always verify reconstitution math against a second source before any dose you actually inject.

How much reconstitution solution a GHK-Cu vial needs

There is no single correct amount. The reconstitution solution is usually bacteriostatic water, though the diluent and volume are set by your product’s own instructions, not by a general rule. What you add is what sets the concentration: vial mg divided by mL of liquid. Adding more makes a weaker mix and a bigger, easier-to-read draw on the syringe. To hit a concentration you have in mind, divide the vial mg by it, so a 50 mg vial at 25 mg/mL needs 2 mL. Below are common GHK-Cu vial sizes at three common volumes (U-100 insulin syringe, where 1 unit = 0.01 mL). These rows are arithmetic only. They do not tell you which diluent, volume, or concentration is appropriate for the product in your hand.

VialReconstitution solutionConcentrationPer insulin unit50 units (0.50 mL) holds
50 mg1 mL50 mg/mL500 mcg25 mg
50 mg2 mL25 mg/mL250 mcg12.50 mg
50 mg3 mL16.67 mg/mL166.67 mcg8.33 mg
100 mg1 mL100 mg/mL1000 mcg50 mg
100 mg2 mL50 mg/mL500 mcg25 mg
100 mg3 mL33.33 mg/mL333.33 mcg16.67 mg
200 mg1 mL200 mg/mL2000 mcg100 mg
200 mg2 mL100 mg/mL1000 mcg50 mg
200 mg3 mL66.67 mg/mL666.67 mcg33.33 mg

Worked example: a 50 mg GHK-Cu vial in 2 mL of bacteriostatic water is 25 mg/mL. One unit on a U-100 pin is 250 mcg, so if your protocol calls for 12.50 mg you would draw to 50 units. That is arithmetic from the numbers you enter, nothing more. Pepdex does not tell you what dose to take.

GHK-Cu reconstitution questions

How do you reconstitute GHK-Cu, and what do you mix it with?
The reconstitution solution is usually bacteriostatic water, but use only the diluent and volume specified in your product's own labeling or dispensing instructions. General technique: add the liquid slowly down the inside wall of the vial, let the powder dissolve on its own without shaking, and keep the mixed vial refrigerated. The amount you add sets the concentration. A 50 mg vial in 2 mL is 25 mg/mL; the same vial in 1 mL is 50 mg/mL. Use the calculator above to run your own numbers.
How much reconstitution solution do you need for a 50 mg GHK-Cu vial?
As much as it takes to reach the concentration you are aiming for, which is why no single number is the answer. Divide the vial mg by your target concentration to get the mL: a 50 mg vial at 50 mg/mL takes 1 mL, at 25 mg/mL takes 2 mL, and at 16.67 mg/mL takes 3 mL. The table above runs the same math for the common GHK-Cu vial sizes (50 mg, 100 mg, 200 mg). Any vial size works in the calculator, including one not listed here. Which concentration is appropriate is set by your product's instructions, not by this page.
How many insulin units is a dose of GHK-Cu?
It depends entirely on how much water you mixed, because milligrams describe the powder and units describe the volume drawn. With a 50 mg vial reconstituted in 2 mL, one unit on a U-100 pin is 250 mcg, so 50 units draws 12.50 mg. Change the water and that number changes. Enter your numbers above to get yours.
What vial sizes does GHK-Cu come in?
Commonly 50 mg, 100 mg, 200 mg. The calculator defaults to 50 mg for GHK-Cu but you can set any vial size. Always work from the mg printed on the vial you actually have, not a number from a forum.
Why is the GHK-Cu vial so much bigger than other peptides?
GHK-Cu is measured in milligrams, not the micrograms typical of other peptides, so its vials are 50 mg and up rather than 5 to 10 mg. That makes the reconstitution numbers run much larger. Enter your vial size and water amount above to see the exact concentration.
Is the GHK-Cu calculator free?
Yes. The reconstitution and units math is completely free with no account. A free account also saves your protocol. The AI Coach and Personal Stack are part of membership.

Educational reference only. Pepdex does not sell peptides, recommend vendors, or tell you what dose to take. GHK-Cu reconstitution math here is unit conversion you can verify by hand. Talk to a qualified provider about your own use.