TB-500 Reconstitution & Dose Calculator
TB-500 is a fragment of Thymosin Beta-4 studied for its role in cell migration and tissue repair. This free calculator turns a TB-500 vial plus bacteriostatic water into the exact insulin syringe units to draw. It is unit-conversion math, not a dosing recommendation. TB-500 vials are commonly 5 mg, 10 mg, so the tool below starts at 5 mg; change any field to match your own vial.
Reconstitution calculator
Pick your peptide, vial size, and BAC water. Get exact units to draw on a 1 mL insulin syringe.

Pick your peptide
Type to search, or pick from the dropdown. Auto-fills the recommended vial size.
- • Standard insulin syringe = 100 units total
- • Small markings = 1 unit each
- • Large markings = 10 units (0.1 mL)
- • 100 units = 1 mL
Always verify reconstitution math against a second source before any dose you actually inject.
TB-500 concentration at a glance
Concentration is just vial mg divided by the bacteriostatic water you add. A more dilute mix gives a bigger, easier-to-read draw on the syringe. Here is a 5 mg TB-500 vial at three common water volumes (U-100 insulin syringe, where 1 unit = 0.01 mL):
| BAC water | Concentration | Per insulin unit | 50 units draws |
|---|---|---|---|
| 1 mL | 5 mg/mL | 50 mcg | 2.50 mg |
| 2 mL | 2.50 mg/mL | 25 mcg | 1.25 mg |
| 3 mL | 1.67 mg/mL | 16.67 mcg | 0.83 mg |
Worked example: a 5 mg TB-500 vial in 2 mL of bacteriostatic water is 2.50 mg/mL. One unit on a U-100 pin is 25 mcg, so if your protocol calls for 1.25 mg you would draw to 50 units. That is arithmetic from the numbers you enter, nothing more. Pepdex does not tell you what dose to take.
TB-500 reconstitution questions
- How do you reconstitute TB-500?
- Add bacteriostatic water to the dry TB-500 vial slowly down the side, let it dissolve without shaking, and store it in the fridge. The amount of water you add sets the concentration. A 5 mg vial in 2 mL of water is 2.50 mg/mL; the same vial in 1 mL is 5 mg/mL. Use the calculator above with your own vial and water to see the exact result.
- How many insulin units is a dose of TB-500?
- It depends entirely on how much water you mixed, because milligrams describe the powder and units describe the volume drawn. With a 5 mg vial reconstituted in 2 mL, one unit on a U-100 pin is 25 mcg, so 50 units draws 1.25 mg. Change the water and that number changes. Enter your numbers above to get yours.
- What vial sizes does TB-500 come in?
- Commonly 5 mg, 10 mg. The calculator defaults to 5 mg for TB-500 but you can set any vial size. Always work from the mg printed on the vial you actually have, not a number from a forum.
- Is TB-500 the same as BPC-157?
- No. TB-500 is a Thymosin Beta-4 fragment studied for body-wide cell migration; BPC-157 is a gastric peptide studied for more local effects. They are different molecules at different vial sizes, so do not reuse one's reconstitution numbers for the other.
- Is the TB-500 calculator free?
- Yes. The reconstitution and units math is completely free with no account. A free account adds saving your protocol plus a 3-day trial of the AI Coach and Personal Stack.
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Educational reference only. Pepdex does not sell peptides, recommend vendors, or tell you what dose to take. TB-500 reconstitution math here is unit conversion you can verify by hand. Talk to a qualified provider about your own use.