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Semaglutide Reconstitution & Dose Calculator

Semaglutide is a GLP-1 receptor agonist, the same molecule sold under the brand names Ozempic and Wegovy. This free calculator turns a Semaglutide vial plus bacteriostatic water into the exact insulin syringe units to draw. It is unit-conversion math, not a dosing recommendation. Semaglutide vials are commonly 5 mg, 10 mg, so the tool below starts at 5 mg; change any field to match your own vial.

Free tool

Reconstitution calculator

Pick your peptide, vial size, and BAC water. Get exact units to draw on a 1 mL insulin syringe.

Educational reconstitution math reference only. This tool does not provide dosage or usage recommendations. Consult a qualified healthcare provider before making any health decisions.
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Type to search, or pick from the dropdown. Auto-fills the recommended vial size.

Your mix
What you mixed
5 mg powder + 2 mL water
Concentration of the liquid
250 mcg per 10 units
Each small line on a 1 mL insulin syringe = 25 mcg
One 5 mg vial lasts you
20days2.9 weeks
At 250 mcg, 1 dose / day, 20 total doses in this vial.
Insulin syringe, draw to the 10 unit mark10 u
0102030405060708090100
Pull the plunger back until the rubber stopper lines up with the 10 mark on the barrel. The red liquid in the picture is the peptide volume.
Syringe unit referenceFull guide →
  • • Standard insulin syringe = 100 units total
  • • Small markings = 1 unit each
  • • Large markings = 10 units (0.1 mL)
  • 100 units = 1 mL

Always verify reconstitution math against a second source before any dose you actually inject.

Semaglutide concentration at a glance

Concentration is just vial mg divided by the bacteriostatic water you add. A more dilute mix gives a bigger, easier-to-read draw on the syringe. Here is a 5 mg Semaglutide vial at three common water volumes (U-100 insulin syringe, where 1 unit = 0.01 mL):

BAC waterConcentrationPer insulin unit50 units draws
1 mL5 mg/mL50 mcg2.50 mg
2 mL2.50 mg/mL25 mcg1.25 mg
3 mL1.67 mg/mL16.67 mcg0.83 mg

Worked example: a 5 mg Semaglutide vial in 2 mL of bacteriostatic water is 2.50 mg/mL. One unit on a U-100 pin is 25 mcg, so if your protocol calls for 1.25 mg you would draw to 50 units. That is arithmetic from the numbers you enter, nothing more. Pepdex does not tell you what dose to take.

Semaglutide reconstitution questions

How do you reconstitute Semaglutide?
Add bacteriostatic water to the dry Semaglutide vial slowly down the side, let it dissolve without shaking, and store it in the fridge. The amount of water you add sets the concentration. A 5 mg vial in 2 mL of water is 2.50 mg/mL; the same vial in 1 mL is 5 mg/mL. Use the calculator above with your own vial and water to see the exact result.
How many insulin units is a dose of Semaglutide?
It depends entirely on how much water you mixed, because milligrams describe the powder and units describe the volume drawn. With a 5 mg vial reconstituted in 2 mL, one unit on a U-100 pin is 25 mcg, so 50 units draws 1.25 mg. Change the water and that number changes. Enter your numbers above to get yours.
What vial sizes does Semaglutide come in?
Commonly 5 mg, 10 mg. The calculator defaults to 5 mg for Semaglutide but you can set any vial size. Always work from the mg printed on the vial you actually have, not a number from a forum.
Is semaglutide the same as Ozempic?
The active molecule is the same. Ozempic and Wegovy are branded semaglutide in a fixed-dose pen. Vial-form semaglutide can be at a different concentration than a branded pen, so always reconstitute from the mg printed on your actual vial rather than assuming a pen's numbers.
Is the Semaglutide calculator free?
Yes. The reconstitution and units math is completely free with no account. A free account adds saving your protocol plus a 3-day trial of the AI Coach and Personal Stack.

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Educational reference only. Pepdex does not sell peptides, recommend vendors, or tell you what dose to take. Semaglutide reconstitution math here is unit conversion you can verify by hand. Talk to a qualified provider about your own use.