Pepdexpepdex
My Stack
№ 053

PE-22-28

Synthetic peptide derived from spadin. TREK-1 channel inhibitor with rapid antidepressant effects in animal models. Pre-clinical.

Nootropic
Evidence: Anecdotal

PE-22-28: Synthetic peptide derived from spadin. TREK-1 channel inhibitor with rapid antidepressant effects in animal models. Pre-clinical. PE-22-28 is derived from spadin and blocks the TREK-1 channel.

FDA ⓘ
Not approved
WADA
Not banned
Typical dose
No standardized human dose
Half-life ⓘ
Short (~hours)
Route ⓘ
Subcutaneous or intranasal
Schedule
Daily
In plain English

PE-22-28 is derived from spadin and blocks the TREK-1 channel. Animal-model antidepressant effects in days rather than weeks. Pre-clinical only, no human dose protocol, real interactions with SSRIs / SNRIs / heart-arrhythmia drugs.

Status & legalityWhat do these mean? →
Natty? ⓘ
Not natty
FDA ⓘ
Not approved

Not FDA approved. Pre-clinical.

Compounding ⓘ
Not classified

Not formally categorized in the FDA bulks lists.

WADA ⓘ
Not listed
Prescribed ⓘ

Not prescribed in conventional medicine.

Who it's for

  • →Researchers studying TREK-1 pathways
  • →Antidepressant-mechanism research
  • →Educational reference, limited human safety data

What to expect

  1. Week 1

    Animal models show mood-task improvements rapidly.

  2. Week 4

    Cycle endpoint in pre-clinical protocols.

  3. Week 8

    No human long term data.

Looking at PE-22-28? Your next 3 steps

  1. 1Work out your exact dose

    Vial size + BAC water turns into the exact units to draw for PE-22-28.

    Open calculator →
  2. 2See what to stack & monitor

    The companion supplements and the bloodwork worth tracking on this kind of protocol.

    Bloodwork guide →
  3. 3Save it & ask the Coach

    A free account saves your stack; membership adds the stack-aware AI Coach.

    Create free account →

How it works (mechanism)

TREK-1 channel inhibitor derived from spadin. TREK-1 is a potassium channel involved in mood regulation; blocking it produces fast antidepressant effects in animal models.

Dosing protocol

Members only

Stacks well with

Members only

Side effects

01Limited human safety data
02Theoretical effects on cardiac TREK-1 channels

When NOT to use

  • ⚠Active SSRI / SNRI / MAOI regimens
  • ⚠Cardiac arrhythmia history
  • ⚠Pregnancy / nursing

Common mistakes

  • • Stacking with other mood-active compounds
  • • Long cycles without safety data
  • • Treating animal-model dose as human dose
Free, no account needed

New to PE-22-28? Grab the starter checklist.

Drop your email and we'll send the one-page first-cycle starter checklist, plus a heads-up if PE-22-28 changes legal status. No account needed.

No spam. No selling your email. Just the checklist and the occasional reclassification alert.

AI Coach, live sample
Members only
is 250mcg of bpc enough for a knee injury?
For a knee, 250 mcg sub-q daily is the standard working dose and a solid place to start. The trick with BPC is consistency, give it weeks, not days. Inject close to the joint, run it 4-6 weeks, and don't drop below 200 mcg, it tends to stop cracking the threshold reliably down there. If it hasn't moved at all by week 3, that's when adding TB-500 earns its place.
how much bac water for a 10mg reta vial?
3 mL is the standard play for a 10 mg reta vial. That's 3,333 mcg/mL, clean unit math across the titration: 2 mg = 60 units, 4 mg = 120 units, 6 mg = 180 units on a 100-unit insulin syringe. Run 2 mL instead if you want fewer, more concentrated shots (5,000 mcg/mL, so 2 mg = 40 units). Since most people titrate up over ~12 weeks, 3 mL keeps the numbers cleanest.
what should i track on bloodwork for tirzepatide?
Lipid panel, ALT/AST (liver enzymes), and an A1C, baseline before you start then every 3 months. If you've got metabolic-syndrome history, add fasting glucose and insulin so you can actually watch insulin sensitivity improve. You don't need a big hormone panel for a GLP-1.

Ask the Coach anything about PE-22-28 or your own stack. This is it working.

Trained only on Pepdex content. Does the dose math, flags interactions, knows your stack. Won't push vendors, won't pretend to be a doctor.

Get the Coach, $7.99/mo →

Frequently asked

What is PE-22-28?+
PE-22-28 is derived from spadin and blocks the TREK-1 channel. Animal-model antidepressant effects in days rather than weeks. Pre-clinical only, no human dose protocol, real interactions with SSRIs / SNRIs / heart-arrhythmia drugs.
Is PE-22-28 FDA approved?+
Not FDA approved. Pre-clinical.
Is PE-22-28 legal?+
PE-22-28 is not FDA-approved. It is sold by compounding pharmacies (with a prescription) and as "research only" by peptide vendors. Possession is generally not criminalized but distribution without authorization may be. Verify local laws.
Is PE-22-28 banned by WADA?+
PE-22-28 is not currently on the WADA prohibited list.
Are you still natty after taking PE-22-28?+
No. PE-22-28 is a performance-enhancing peptide and would disqualify a strict natty claim.
Do doctors prescribe PE-22-28?+
Not prescribed in conventional medicine.
What's the typical dose of PE-22-28?+
No standardized human dose. Animal models use per-kg dosing.
What are the side effects of PE-22-28?+
Common side effects include: Limited human safety data; Theoretical effects on cardiac TREK-1 channels. Less common effects and full safety details are on the entry page.
How long until PE-22-28 starts working?+
Animal models show mood-task improvements rapidly.
What can you stack with PE-22-28?+
Common pairings: Selank or Semax for compounded mood/anxiolytic effects (community pattern); Standalone. Full stacking protocol and timing on the entry page.
Where do people get PE-22-28?+
PE-22-28 is most commonly sold by research-only peptide vendors and by compounding pharmacies (the latter requires a prescription). Pepdex is not a vendor, see /coa for how to verify a Certificate of Analysis before buying from any source, and /guides/scam-vendor-spotting for vendor red flags.

More in Nootropic

№ 033
Oxytocin

The 'bonding hormone' you make naturally. Synthetic oxytocin is FDA-approved for labor; off-label use covers social, anxiety, and intimacy contexts.

№ 047
Cerebrolysin

A brain-recovery drug used abroad for stroke, head injury, and Alzheimer's. A porcine brain-derived peptide complex used clinically in 50+ countries (Russia, China, Mexico, EU) for stroke recovery, TBI, and Alzheimer's. It's a mixture of low-molecular-weight peptides, not a single compound.

№ 030
Semax

For alertness and focus. A Russian-origin nootropic peptide on the alert/focus end of the spectrum that pairs with Selank for a balanced cognitive stack.

№ 041
Dihexa

A brain compound aimed at building new connections between neurons. An angiotensin IV analog with strong synaptogenic effect in animal models, often-cited as 'BDNF on steroids.' Very limited human data.